HPLC Method for Analysis of 4-Amino-2-nitrophenol on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Amino-2-nitrophenol · 4-Amino-2-nitrophenol is an organic compound with the molecular formula C₆H₆N₂O₃ . · Properties: Appearance: Typically a yellow to orange crystalline solid · Molecular weight: ~154.12 g/mol · Solubility: Soluble in water and polar organic solvents · Uses: Intermediate in the manufacture of dyes, pigments, and pharmaceuticals
4-Amino-2-nitrophenol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 275 nm.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| LOD | 93 ppb |
| Class of Compounds | Aromatic amine |
| Analyzing Compounds | 4-Amino-2-nitrophenol |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended