HPLC MS Method for the Analysis of Cytokinins: A Phytohormone Class and Brassinazole on Primesep 200 Column
https://sielc.com/hplc-ms-method-cytokininsChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
HPLC Method for Analysis of trans-Zeatin , Kinetin , N6-(Δ2-Isopentenyl)adenine , Thidiazuron , 6-Benzylaminopurine , Brassinazole on Primesep 200 Column
It looks like you’re listing a series of compounds, most of which are cytokinins or related plant hormones. Here’s a brief overview and classification for the compounds you mentioned:
trans-Zeatin : · Kinetin : · N6-(Δ2-Isopentenyl)adenine (iP): · N6-Benzyladenine (BA):
Method Parameters
| Mobile Phase | MeCN – 20%, 4 min than Gradient MeCN/H2O – 20-90% 16 min |
|---|---|
| Buffer | Formic Acid -0.2% |
| Flow Rate | 0.2 mg/ml |
| Detection | UV 290 nm, 265 nm ESI SIM 220+, 216+, 204+,226+, 328+, 221+ |
| Class of Compounds | Phytohormone |
| Analyzing Compounds | trans-Zeatin,Kinetin,N6-(Δ2-Isopentenyl)adenine,Thidiazuron,6-Benzylaminopurine,Brassinazole |
HPLC Column Used
Primesep 200, 2.1 x 100 mm, 5 µm, 100 A, dual ended