HPLC MS Method for Analysis of Trimellitic anhydride on Chromni Column

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Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Trimellitic anhydride

Trimellitic anhydride is a trifunctional, highly reactive aromatic anhydride with the chemical formula C 9 H 4 O 5 . It is used as a curing agent for epoxy and other resins; in vinyl plasticizers, paints, coatings, dyes, pigments and a wide variety of other manufactured products. It is a common precursor to plasticizers for polyvinyl chloride (PVC). In acidic water, Trimellitic Anhydride will ‘open up’ back into Trimellitic Acid . You can find detailed UV spectra of Trimellitic anhydride and information about its various lambda maxima by visiting the following link.

Trimellitic anhydride is analyzed using a Chromni stationary phase column under gradient conditions with a mobile phase composed of acetonitrile (MeCN) and water. Detection is performed by UV absorbance detection.

Method Parameters

Mobile PhaseMeCN – 99.5%
BufferFormic Acid – 0.5%
Flow Rate0.2 mL/min
DetectionUV 263 nm,  ESI-SIM: [M-H]1-191
Limit of Detection*263 nm – 7 ppb, LC MS – 50 ppb
Class of CompoundsOrganic Compound
Analyzing CompoundsTrimellitic anhydride

HPLC Column Used

Chromni, 2.1 x 150 mm, 3 µm, 100 A, surface coated