HPLC MS Method for Analysis of Oligonucleotide 8-mer on Chromni Column

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Chromatogram

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Oligonucleotides

Oligonucleotides are short sequences of nucleotides that serve as essential tools in molecular biology. These oligonucleotides can be composed of any combination of the four standard nucleotides: adenine (A), thymine (T), cytosine (C), and guanine (G).

Research and Studies : · Medical and Diagnostic Applications : · Educational Purposes : · Demonstrating basic principles of nucleic acid chemistry and genetics. · Nanotechnology :

Method Parameters

Mobile PhaseGradient MeCN – 70 – 50%, 15 min
BufferAmmonium Acetate pH 5.0 – 5mM
Flow Rate0.2 ml/min
DetectionLC-MS
Class of CompoundsOligonucleotide
Analyzing CompoundsOligonucleotides

HPLC Column Used

Chromni, 2.1 x 150 mm, 3 µm, 100 A, surface coated
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