HPLC Method for Analysis of Gedatolisib on Chromni Column
https://sielc.com/hplc-ms-analysis-of-gedatolisibChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Gedatolisib
Gedatolisib is an anti-cancer medication with the chemical formula C 32 H 41 N 9 O 4 . It is used as treatment for breast cancer. It works as a kinase inhibitor through binding the different p110 catalytic subunit isoforms of PI3K and the kinase site of mTOR
Gedatolisib can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an sulfuric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN – 90 – 70%, 10 min |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm, 320 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Gedatolisib |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated