HPLC MS Method for Separation of Cyclosporin B on Primesep 200 Column
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Description
· HPLC Method for Analysis of Cyclosporin B in on Primesep 200 Column
Cyclosporine B is a calcineurin inhibitor and analogue of cyclosporin A, with the molecular formula C 61 H 109 N 11 O 12 . It is used primarily as a an immunosuppressant medication with antifungal activities. It is said to work inhibiting P-glycoprotein activity and suppressing the growth of T-lymphoma cell lines. You can find detailed UV spectra of Cyclosporine B and information about its various lambda maxima by visiting the following link .
Cyclosporin B can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, and Ammonium Formate. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 10 mM |
| Flow Rate | 0.5 ml/min |
| Detection | ESI-SIM: [M+H]+m/z 1189 |
| Column Temperature | 60 C |
| LOD* | 55 ppb |
| Class of Compounds | Drug |
| Analyzing Compounds | Cyclosporin B |