HPLC MS Method for Analysis of Acetazolamide in Pharmaceutical Formulation on Primesep 100 Column
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Description
· HPLC Method for Analysis of Acetazolamide in on Primesep 100 Column
Acetazolamide is a chiral aromatic amine with the molecular formula C 4 H 6 N 4 O 3 S 2 . It is used primarily to treat glaucoma, epilepsy, acute mountain sickness, heart failure, and more. It works as a carbonic anhydrase inhibitor, which causes the accumulation of carbonic acid, which is found in a wide variety of tissue. It is on the World Health Organization’s List of Essential Medicines. You can find detailed UV spectra of [ compounds ] and information about its various lambda maxima by visiting the following link .
Acetazolamide can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, and Ammonium Formate. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O 5/95% |
|---|---|
| Buffer | Ammonium formate pH 3.0 – 10 mM |
| Flow Rate | 0.5 ml/min |
| Detection | LC MS ESI SIM 223 (M+H)+, UV 266 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Acetazolamide |