HPLC Method for Analysis of Thidiazuron on Primesep 100 Column
https://sielc.com/hplc-method-thidiazuronChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode SIELC Technologies · HPLC Method for Analysis of Thidiazuron on Primesep 100 Column · Thidiazuron (TDZ)
A phenylurea derivative that acts as a cytokinin-like compound. Widely used in plant tissue culture to promote shoot regeneration and induce cytokinin-like effects.
Thidiazuron can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.
You can find detailed UV spectra of Thidiazuron and information about its various lambda maxima by visiting the following link .
Method Parameters
| Mobile Phase | MeCN – 40% |
|---|---|
| Buffer | H2SO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 201 nm |
| Sample | 0.01 mg/ml |
| Diluent | MeCN/H2O- 50/50% |
| LOD* | 5 ppb |
| Class of Compounds | Phytohormone |
| Analyzing Compounds | Thidiazuron |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended