HPLC Method for Analysis of Tribenzylamine (TBA)  on Primesep B Column

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Chromatogram

Description

Tribenzylamine , also written as TBA , is an organic compound with the chemical formula C 21 H 21 N . It falls under the class of triarylamines. Triarylamines are compounds containing an amine group (-NH2) attached to three aromatic groups (in this case, benzyl groups). In the case of tribenzylamine, the amine nitrogen is attached to three benzyl groups, which are phenyl (benzene) rings attached to a methylene (-CH2-) group. It’s often used in organic chemistry as a reagent, particularly in the production of other complex organic compounds.

The Tribenzylamine can be retained and analyzed using a mixed-mode Primesep B column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Sulfuric acid, which serves as a buffer. This analytical method can be monitored using UV detection at 200 nm.

Method Parameters

Mobile PhaseMeCN -10%
BufferH2SO4 – 0.1%
Flow Rate0.5 ml/min
DetectionUV 200 nm
Class of CompoundsTriarylamines
Analyzing CompoundsTribenzylamine

HPLC Column Used

Primesep B, 3.2 x 100 mm, 5 µm, 100 A, dual ended