HPLC Method for Separation PEI and PVP on PVP Column
https://sielc.com/hplc-method-pvp-peiChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyvinylpyrrolidone(PVP) , Polyethylenimine PEI
Polyvinylpyrrolidone(PVP) , Polyethylenimine PEI can be retained and analyzed using the PVP column. The analysis utilizes an step gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate. Detection is performed using ELSD.
Method Parameters
| Mobile Phase | Step gradient MeCN/H2O |
|---|---|
| Buffer | Ammonium Acetate pH 4.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD |
| LOD | PVP 0.5 ppm, PEI 1.7 ppm |
| Class of Compounds | Polymer |
| Analyzing Compounds | Polyvinylpyrrolidone(PVP),Polyethylenimine PEI |
HPLC Column Used
PVP, 2.1 x 50 mm, 5 µm, 100 A, dual ended