HPLC Method for Separation Povidone-iodine (PVP-I) on PVP Column

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of Iodide , Polyvinylpyrrolidone(PVP)

Polidone Iodide (Povidone-Iodine / PVP-I) Polidone Iodide, also known as Povidone-Iodine (PVP-I), is a widely used antiseptic made from iodine and polyvinylpyrrolidone (PVP). This combination allows for a slow, sustained release of iodine, enhancing antimicrobial effects while reducing irritation.

PVP (Polyvinylpyrrolidone): A water-soluble synthetic polymer that acts as: · It helps form a stable complex with iodine, improving its medical use. · Iodine: The active antimicrobial agent, effective against: · Slow release from the PVP complex ensures long-lasting, gentle antiseptic action.

Iodide , Polyvinylpyrrolidone(PVP) can be retained and analyzed using the PVP column. The analysis utilizes an step gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate. Detection is performed using ELSD.

Method Parameters

HPLC Column Used