HPLC Method for Analysis of Chromium(III) on BIST B+ Column
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Description
· HPLC Method for Analysis of Chromium(III) on BIST B+ by SIELC Technologies
Chromium(III) is a nutrient with the chemical formula Cr 3+ . It is believed to be essential for human metabolism of insulin, sugar, and lipids. Though, the notion is currently contested. It can be found naturally in rocks, soil, plants, as well as various animal products.
Using SIELC’s newly introduced BIST™ method, Chromium(III) can be retained on a positively-charged anion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged trivalent Chromium ion to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Chromium(III) can be separated, retained, and UV detected at 200 nm.
Method Parameters
| Mobile Phase | Gradient MeCN – 60-20%, 10 min |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Peak Retention Time | 5.71 min |
| LOD | 0.4 ppm |
| Class of Compounds | Ion |
| Analyzing Compounds | Chromium(III) |