HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on BIST B+ Column
https://sielc.com/hplc-method-of-carnosineChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Carnosine (beta-alanyl-L-histidine) .
Carnosine is a dipeptide molecule with the chemical formula C 9 H 14 N 4 O 3 . It is synthesized from beta-alanine and histidine. It is a naturally occurring pH buffer. It is found in muscles and brain tissue, but is produced in the liver. Some use it as a nutritional supplement, but it can be used in topical applications to reduce wrinkles.
Using SIELC’s newly introduced BIST™ method, Carnosine (beta-alanyl-L-histidine) can be retained on a positively charged, antion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged peptide to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Carnosine can be separated, retained, and UV detected at 205 nm.
Method Parameters
| Mobile Phase | MeCN – 75% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 205 nm |
| Peak Retention Time | 3.71 min |
| Class of Compounds | Dipeptide |
| Analyzing Compounds | Carnosine (beta-alanyl-L-histidine) |