HPLC – MS Method for Analysis of Paraquat on Obelisc R Column
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Description
· HPLC Method for Analysis of Paraquat on Obelisc R Column by SIELC Technologies
Paraquat is a widely used, non-selective herbicide known for its high efficacy in controlling a wide range of weeds. It has been an important tool in agriculture since its introduction in the mid-20th century. However, its use is accompanied by significant safety concerns due to its high toxicity.
Paraquat can be retained and analyzed on a Obelisc R mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)
Method Parameters
| Mobile Phase | MeCN – 50%, |
|---|---|
| Buffer | Ammonium Formate pH 3.0-50 mM |
| Flow Rate | 0.2 ml/min |
| Detection | SIM 186 +, UV 259 nm |
| Spray Voltage: | 1.5 kV |
| Nebulizing gas: | 1.5 L/min |
| Drying gas: | 15 L/min |
| DL temp: | 250 ˚C |
| Heat Block: | 400 ˚C |
| Class of Compounds | Pesticides |
| Analyzing Compounds | Paraquat |
HPLC Column Used
Obelisc R, 2.1 x 100 mm, 5 µm, 100 A, dual ended