HPLC Method for Analysis of Methionine on Primesep 100 Column

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Chromatogram

Description

· Separation type: Liquid Chromatography Mixed-mode SIELC Technologies · HPLC Method for Analysis of Methionine on Primesep 100 Column

Methionine is an essential amino acid, meaning the body cannot synthesize it, so it must be obtained through the diet. It’s crucial for many bodily functions, including:

Protein Synthesis: Methionine is a building block for proteins.

Methylation: It acts as a precursor to S-adenosylmethionine (SAMe), which is involved in methylation processes that are vital for DNA regulation, detoxification, and neurotransmitter production.

Antioxidant Function: Methionine contributes to the synthesis of cysteine, which is a precursor to glutathione, one of the body’s most important antioxidants.

Liver Health: It’s also important for liver function and fat metabolism.

Method Parameters

Mobile PhaseMeCN – 35%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Limit of Detection40 ppb
Class of CompoundsHerbicide
Analyzing CompoundsMethionine

HPLC Column Used

Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended