HPLC Method for Analysis of Kynurenine on Primesep 100 Column
https://sielc.com/hplc-method-kynurenineChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode SIELC Technologies · HPLC Method for Analysis of Kynurenine on Primesep 100 Column
Kynurenine is a key metabolite in the tryptophan catabolic pathway, often referred to as the kynurenine pathway. This pathway is responsible for breaking down the essential amino acid tryptophan into several bioactive compounds.
Kynurenine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.
You can find detailed UV spectra of Kynurenine and information about its various lambda maxima by visiting the following link .
Method Parameters
| Mobile Phase | MeCN – 35% |
|---|---|
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Samples | 0.5 mg/ml in H2O – 100% |
| Injection volume | 1 µl |
| LOD* | 20 ppb (250 nm) |
| Class of Compounds | Kynurenines |
| Analyzing Compounds | Kynurenine |