HPLC Method for Analysis of Isonicotinic Acid on Primesep 100 Column
https://sielc.com/hplc-method-isonicotinic-acidChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode SIELC Technologies · HPLC Method for Analysis of Isonicotinic Acid on Primesep 100 Column
Isonicotinic Acid can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.
You can find detailed UV spectra of Isonicotinic Acid and information about its various lambda maxima by visiting the following link .
Method Parameters
| Mobile Phase | MeCN – 10% |
|---|---|
| Buffer | H2SO4 -0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Samples | 1.0 mg/ml in MeCN/H2O – 50/50% |
| Injection volume | 1 µl |
| LOD* | 2 ppb (200 nm) |
| Class of Compounds | Acid |
| Analyzing Compounds | Isonicotinic Acid |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended