HPLC UV Method for Separation of Nicotinic Acid and Pantothenic Acid on Chromni Column

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinic Acid (Vitamin B3) , Pantothenic Acid (Vitamin B5) .

Nicotinic acid , also known as niacin and vitamin B3 , is a water-soluble B vitamin with C₆H₅NO₂ molecular formula. It is an essential nutrient that is crucial for energy production. Nicotinic acid is commonly used as a dietary supplement to treat vitamin B3 deficiency, and it’s also prescribed in higher doses to help lower cholesterol levels. It works by dilating blood vessels, which can lead to a flushing sensation, hence it is sometimes referred to as “flushing niacin.” You can find detailed UV spectra of Niacin and information about its various lambda maxima by visiting the following link.

Pantothenic Acid , also known as vitamin B5 , is an essential nutrient with the chemical formula C 19 H 17 NO 5 . It plays a crucial role in energy and coenzyme A production. That energy and coenzyme are used for synthesis and degradation of proteins, carbohydrates, and fats. You can find detailed UV spectra of Nicotinic Acid (Vitamin B3) , Pantothenic Acid (Vitamin B5) and information about its various lambda maxima by visiting the following link.

Nicotinic Acid (Vitamin B3) , Pantothenic Acid (Vitamin B5) can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and water with a phosphoric acid buffer. Detection is performed using UV.

Method Parameters

HPLC Column Used