HPLC Method for Simultaneous Determination of Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate on Primesep 100 Column

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinic Acid/Niacin (3-pyridinecarboxylic acid) , Nicotinamide , Pyridinedicarboxylic Acid , 2,5-Pyridinedicarboxylic acid , 5-Ethyl-2-methylpyridine , Ethyl nicotinate .

2,5-Pyridinedicarboxylic acid ( PDA ), also known as Dipicolinic acid ( DPA ), is a key factor in bacterial endospore heat resistance. It also plays an important role in preparing transition metal complexes for ion chromatography.

Nicotinic acid , also known as niacin , is a water-soluble B vitamin with C 6 H 5 NO 2 molecular formula. It is an essential nutrient that plants and animals synthesize from tryptophan. It is also used as a dietary supplement to treat pellagra, which is characterized by dermatitis of sunlight-exposed skin and is said to be caused by a corn-based diet. Nicotinic acid was found on asteroids and meteorites.

Nicotinamide , also known as niacinaminde , is a form of vitamin B 3 with the chemical formula C 6 H 6 N 2 O 2 . It is preferred treatment for pellagra and is a common treatment for acne due to it’s anti-inflammatory properties. If consumed daily, it is shown to decrease the risk of skin cancers other than melanoma. It is a derivative of Nicotinic acid and is a key component of the coenzymes nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP+). You can find detailed UV spectra of Nicotinamide and information about its various lambda maxima by visiting the following link.

Ethyl nicotinate , also known as nicotine acid ethyl ester , has the chemical formula C 8 H 9 NO 2 . It is a popular topical treatment for ligament, joint, and muscle pain.

5-Ethyl-2-methylpyridine is a common precursor to Nicotinic acid with the chemical formula C 8 H 11 N . It is produced by condensation of paraldehyde and ammonia.

Nicotinic Acid/Niacin (3-pyridinecarboxylic acid) , Nicotinamide , Pyridinedicarboxylic Acid , 2,5-Pyridinedicarboxylic acid , 5-Ethyl-2-methylpyridine , Ethyl nicotinate can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an analytical method with a simple gradient mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 250 nm with high resolution and peak symmetry.

Method Parameters

HPLC Column Used