HPLC Method for Determination of Vecuronium on Primesep SB Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Vecuronium bromide
Vecuronium bromide , also known as Norcuron , is a muscle relaxant with the chemical formula C 34 H 57 BrN 2 O 4 . It is primarily used during surgery and executions by lethal injection. Reversal of vecuronium’s effects can be achieved through administration sugammadex.
HPLC methods for Vecuronium bromide are of great interest to pharmaceutical research and industry. Vecuronium bromide can be separated and retained on a mixed-mode stationary phase Primesep SB column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) ionic modifier. This analysis method can be UV detected at 260 nm with high resolution and peak symmetry and is compatible with Mass Spectrometry (MS), ELSD, and CAD.
Method Parameters
| Mobile Phase | MeCN – 10% |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 50 mM, 100 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD, 50C |
| Class of Compounds | Drug |
| Analyzing Compounds | Vecuronium bromide |