HPLC UV Method for Analysis of N-(Phosphonomethyl)iminodiacetic acid (PMIDA) on Newcrom BH Column
https://sielc.com/hplc-method-for-pmidaChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of PMIDA (Phosphonomethyliminodiacetic acid) .
N-(Phosphonomethyl)iminodiacetic acid , also known as PMIDA , is a key intermediate in the making of glyphosate. It has the chemical formula (HO) 2 P(O)CH 2 N(CH 2 CO 2 H) 2 . You can find detailed UV spectra of PMIDA and information about its various lambda maxima by visiting the following link.
PMIDA (Phosphonomethyliminodiacetic acid) can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | Gradient H3PO4 – 0.5-2.0%, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| LOD* | 6 ppm |
| Class of Compounds | Herbicide |
| Analyzing Compounds | PMIDA (Phosphonomethyliminodiacetic acid) |
HPLC Column Used
Newcrom BH, 4.6 x 100 mm, 5 µm, 100 A, dual ended