HPLC LC MS Method for Analysis of Thiamine on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine) .
Thiamine , also known as vitamin B1 , is an essential nutrient with the chemical formula C 12 H 17 ClN 4 OS . It plays a crucial role in energy metabolism and the proper functioning of the nervous system. It helps the body convert carbohydrates into energy and is vital for the growth, development, and function of cells. You can find detailed UV spectra of Vitamin B1 (Thiamine) and information about its various lambda maxima by visiting the following link.
Vitamin B1 (Thiamine) can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using LC MS.
Method Parameters
| Mobile Phase | Gradient MeCN/H2O – 70/30 – 40/60%, 10 min |
|---|---|
| Buffer | Ammonium formate pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ESI-SIM: [M+2H]2+265.1 |
| Limit Of Detection* | 4 ppb |
| Class of Compounds | Vitamin |
| Analyzing Compounds | Vitamin B1 (Thiamine) |