HPLC UV Method for Simultaneous Analysis of Glyphosate and N-(Phosphonomethyl)iminodiacetic acid (PMIDA) on Newcrom BH Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of PMIDA (Phosphonomethyliminodiacetic acid) , Glyphosate .
Glyphosate is a broad-spectrum herbicide. It works through inhibiting the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase. The enzyme is essential for producing amino acids within the plant. Glyphosate is used across agriculture and forestry, as well as rare aquatic environments. It’s chemical formula is C 3 H 8 NO 5 P . You can find detailed UV spectra of Glyphosate and information about its various lambda maxima by visiting the following link.
N-(Phosphonomethyl)iminodiacetic acid , also known as PMIDA , is a key intermediate in the making of glyphosate. It has the chemical formula (HO) 2 P(O)CH 2 N(CH 2 CO 2 H) 2 . You can find detailed UV spectra of PMIDA and information about its various lambda maxima by visiting the following link.
PMIDA (Phosphonomethyliminodiacetic acid) , Glyphosate can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | Gradient H3PO4 – 0.1-1%, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| LOD* | 4 ppm |
| Class of Compounds | Herbidices |
| Analyzing Compounds | PMIDA (Phosphonomethyliminodiacetic acid),Glyphosate |