HPLC Chiral Separation of Fipronil Enantiomers on Cyralis CM Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Fipronil .

Fipronil is an insecticide with the chemical formula C 12 H 4 Cl 2 F 6 N 4 OS . It works through causing hyperexcitation of the nerves and muscles in contaminated insects.  In Texas, it is often used to control invasive species of ants and wasps. It is also an active ingredient in flea and tick treatments for pets. WHO classifies it as a Class II, moderately hazardous, pesticide.

You can find detailed UV spectra of Fipronil and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Fipronil enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Fipronil can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Method Parameters

HPLC Column Used