HPLC Method for Determination of Triazolam on Newcrom R1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Triazolam .
Triazolam is a prescription-strength benzodiazepine with the chemical formula C 17 H 12 Cl 2 N 4 . It is used to treat insomnia, as it is a central nervous system depressant tranquilizer. Compared to other benzodiazepines, it is fairly obscure, preventing misuse and abuse of it. It is a Schedule IV drug.
Triazolam can be retained and analyzed on a Newcrom R1 reverse-phase column using a simple isocratic analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN – 35% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Triazolam |
HPLC Column Used
Newcrom R1, 2.1 x 100 mm, 5 µm, 100 A, dual ended