HPLC Method for Determination of Delafloxacin on Primesep 100 Column
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Description
Delafloxacin , also known as Baxdela , is a popular antibiotic with the chemical formula C 18 H 12 ClF 3 N 4 O 4 . It is used to treat skin infections from both gram-positive and gram-negative bacteria.
Delafloxacin can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 290 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN – 40% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 290 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Delafloxacin |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended