HPLC Method for Determination of Borate Ion in Boric Acid and Borax on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Orthoborate .
Sodium , which has a chemical symbol of Na , is a soft alkali metal that is highly reactive. It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial for the body’s function and fluid balance.
Borate ion , also known as Orthoborate , is a polyatomic anion with the formula BO 3 3- . It is used primarily in the making of boric acid and orthoborate salts.
Orthoborate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using ELSD.
Method Parameters
| Mobile Phase | MeCN/H2O – 90/10 |
|---|---|
| Buffer | AmFM pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Class of Compounds | Ions |
| Analyzing Compounds | Orthoborate |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated