HPLC Method for Determination of Micafungin on Newcrom R1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Micafungin
Micafungin is an anti-fungal medication with the chemical formula C 56 H 71 N 9 O 23 S . It is used to treat infections including but not limited to candidemia, abscesses, and esophageal candidiasis. It works through inhibiting the production of beta-1,3-glucan. It is only accessible through perscription.
Micafungin can be retained and analyzed on a Newcrom R1 reverse-phase column using a simple isocratic analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 270 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN – 45% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 270 nm |
| LOD | 0.02 ppm |
| Class of Compounds | Drug |
| Analyzing Compounds | Micafungin |
HPLC Column Used
Newcrom R1, 2.1 x 100 mm, 3 µm, 100 A, dual ended