HPLC Method for Determination of Azithromycin on Primesep 200 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Azithromycin .
Azithromycin is an antibiotic with the chemical formula C 38 H 72 N 2 O 12 . It is used in the treatment of various bacterial infections including but not limited to pneumonia, strep throat, and STI. As a medication, it is available in a variety of forms: tablets, capsules, injections, and solutions. It is on the WHOs list of essential medicines and is classified as critically important.
In reversed-phase HPLC, it generally exhibits significant tailing. The tailing can be substantially reduced by using a mixed-mode Primesep 200 column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.
Method Parameters
| Mobile Phase | MeCN – 75% |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 50 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD, 50C |
| Class of Compounds | Drug |
| Analyzing Compounds | Azithromycin |
HPLC Column Used
Primesep 200, 2.1 x 50 mm, 5 µm, 100 A, dual ended