HPLC Method for Analysis of Citrulline on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Citrulline .
Citrulline is an alpha-amino acid with the chemical formula C 6 H 13 N 3 O 3 . It is a key intermediate in the urea cycle. It can be derived from a variety of sources, such as arginine, ornithine, and asymmetric dimethylarginine. Commercially, it is used as a dietary supplemant as L-citrulline boots the production of nitric oxide, which helps with arterial function.
Citrulline can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | H2SO4 – 0.1% |
|---|---|
| Buffer | MeCN/H2O – 10/90% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |
| LOD* | 0.3 ppm |
| Class of Compounds | Amino Acid |
| Analyzing Compounds | Citrulline |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended