HPLC Method for Analysis of Bivalirudin and Asp9 – bivalirudin on BIST A+
https://sielc.com/hplc-method-for-bivalirudinChromatogram
Description
· HPLC Method for Analysis of Bivalirudin on BIST A+ Column by SIELC Technologies
Bivalirudin is a short, 21-amino-acid peptide with the chemical formula C 98 H 138 N 24 O 33 . It has 2 basic and 6 acidic groups. It is sold as a direct thrombin inhibitor (DTI), which is used as an anticoagulant.
Asp 9 -bivalirudin is a process impurity and can be a result of the degradation of the drug bivalirudin. It has the chemical formula C 98 H 137 N 23 O 34 .
SIELC’s new BIST™ mode can retain and separate Bivalirudin and its degradant with a TMDAP phosphate buffer, and the respective retention times can be controlled by altering the pH of the buffer.
Method Parameters
| Mobile Phase | MeCN – 30% |
|---|---|
| Buffer | TMDAP phosphate – 5 mM pH 5.0, pH 6.0 |
| Flow Rate | 0.2 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Drugs, Anticoagulant |
| Analyzing Compounds | Bivalirudin |
HPLC Column Used
BIST A+, 2.1 x 100 mm, 3 µm, 100 A, dual ended