HPLC-MS Method for Analysis of Lipid Standards: Triglyceride Mixtures on Lipak Column
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HPLC-MS Method for Analysis of Lipid Standards: Triglyceride Mixtures on Lipak Column
Description
· HPLC-MS Method for Analysis of Lipid Standards: Triglyceride Mixtures on Lipak Column · Series of triglycerides, which are esters derived from glycerol and three fatty acids.
Tricaprylin , Tricaprin , Trimyristin , Trilaurin , Tripalmitin can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of methanol (MeOH), ethanol (EtOH), ammonium formate and formic acid as a buffer. Detection is achieved using LC MS.
Method Parameters
| Mobile Phase | Gradient MeOH/EtOH – 60/40-10/90% in 10 min |
|---|---|
| Buffer | AmFm– 10 mM, FA – 0.05% |
| Flow Rate | 0.5 ml/min |
| Detection | ESI-SIM: [M+NH4]⁺ – (488, 530, 656, 741, 825);[M + HCOOH + H]⁺ – (685, 853) |
| Class of Compounds | Lipid Standards: Triglyceride Mixtures |
| Analyzing Compounds | Tricaprylin,Tricaprin,Trimyristin,Trilaurin,Tripalmitin |
HPLC Column Used
Lipak, 3.2 x 100 mm, 5 µm, 100 A, dual ended