HPLC ELSD Method for Analysis Trifluoroacetic acid on Primesep B4 Column
https://sielc.com/hplc-method-for-analysis-tfaChromatogram
Description
· HPLC Method for Analysis of TFA (Trifluoroacetic Acid) on Primesep B4 Column by SIELC Technologies · Trifluoroacetic acid (TFA) is a strong organic acid with the chemical formula CF₃COOH. · Properties: · Uses: · Precautions:
Trifluoroacetic acid can be retained and analyzed using an Primesep B4 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD
Method Parameters
| Mobile Phase | MeCN/H2O – 70/30% |
|---|---|
| Buffer | Ammonium formate pH 3.0 – 60 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C,with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Samples | 1 mg/ml |
| Injection volume | 3 µl |
| LOD* | 1 ppm |
| Class of Compounds | Quaternity amines |
| Analyzing Compounds | TFA (Trifluoroacetic Acid) |
HPLC Column Used
Primesep B4, 4.6 x 150 mm, 5 µm, 100 A, dual ended