HPLC ELSD Method for Analysis of Spermidine on Obelisc R Column
https://sielc.com/hplc-method-for-analysis-spermidineChromatogram
Description
· HPLC Method for Analysis of Spermidine on Obelisc R Column by SIELC Technologies
Spermidine is a polyamine compound with the chemical formula C 7 H 19 N 3 that is found in all living cells. It is a biogenic amine and is involved in various cellular processes.
Biological Roles: · Natural Sources: · Research and Health Implications: · Supplementation:
You can find detailed UV spectra of Spermidine and information about its various lambda maxima by visiting the following link.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | Ammonium formate pH 3.0 – 30 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C,with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Samples | 0.1 mg/ml |
| Injection volume | 5 µl |
| LOD* | 200 ppb |
| Class of Compounds | Polyamines |
| Analyzing Compounds | Spermidine |
HPLC Column Used
Obelisc R, 2.1 x 100 mm, 5 µm, 100 A, dual ended