HPLC Method for Analysis of Sofosbuvir on Primesep B Column
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Description
· HPLC Method for Analysis of Sofosbuvir on Primesep B Column by SIELC Technologies
Sofosbuvir is an oral antiviral medication used to treat hepatitis C virus (HCV) infection. It is a nucleotide analog inhibitor that targets the HCV NS5B RNA polymerase, preventing viral replication. Often combined with other antiviral drugs, sofosbuvir has revolutionized HCV treatment by offering a shorter duration of therapy, higher cure rates, and minimal resistance, with fewer side effects compared to older treatments.
Sofosbuvir can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 260 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| LOD | 70.3 ppb |
| Class of Compounds | Nucleotide Analog Prodrug |
| Analyzing Compounds | Sofosbuvir |