HPLC Method for Analysis of Semaglutide on Primesep SB Column
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Description
· HPLC Method for Analysis of Semaglutide on Primesep SB Column by SIELC Technologies
Semaglutide is a GLP-1 receptor agonist used for managing type 2 diabetes and obesity. It mimics the effects of the natural hormone GLP-1, promoting insulin secretion, reducing glucagon levels, slowing gastric emptying, and suppressing appetite. These mechanisms help control blood sugar levels and support weight loss.
Semaglutide can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and TFA. Detection is performed using UV at 280 nm.
Method Parameters
| Mobile Phase | Gradient MeCN 30-70%, 10 min |
|---|---|
| Buffer | TFA – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 280 nm |
| LOD | 59.4 ppb |
| Class of Compounds | GLP-1 Receptor Agonist |
| Analyzing Compounds | Semaglutide |
HPLC Column Used
Primesep SB, 3.2 x 100 mm, 3 µm, 100 A, dual ended