HPLC Method for Analysis of p-Phenylenediamine on Primesep 100 Column
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Description
· HPLC Method for Analysis of p-Phenylenediamine on Primesep 100 Column by SIELC Technologies
p-Phenylenediamine , commonly known as PPD , is an organic compound with the formula C 6 H 8 N 2 . used in hair dyes and black rubber products, especially kevlar and twaron.It’s use in hair dyes is being phased out, however, in favor of other aniline analogues. You can find detailed UV spectra of p-Phenylenediamine and information about its various lambda maxima by visiting the following link .
p-Phenylenediamine be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Samples | p-Phenylenediamine (1.3 mg/mL) |
| Injection volume | 3 µl |
| Class of Compounds | Aromatic amines |
| Analyzing Compounds | p-Phenylenediamine |