HPLC MS Method for Analysis of Zidesamtinib on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Zidesamtinib .
Zidesamtinib is an anti-cancer medication with the chemical formula C 22 H 22 FN 7 O . It is primarily used to treat non-small cell lung cancer and works as a kinase inhibitor.
Zidesamtinib can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using LC MS.
Method Parameters
| Mobile Phase | Gradient MeCN/H2O |
|---|---|
| Buffer | AmAc pH 5.0 – 10 mM |
| Flow Rate | 0.5 ml/min |
| Detection | LC MS ESI SIM 420 [M+H]+ |
| Class of Compounds | Drugs |
| Analyzing Compounds | Zidesamtinib |
HPLC Column Used
Chromni, 3.2 x 100 mm, 3 µm, 100 A, dual ended