HPLC Method for Analysis of Uridine on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Uridine · Uridine is a glycosylated pyrimidine analog with the molecular formula C 9 H 12 O 6 . · Properties: Appearance: Typically a white or off-white powder. · Molecular weight: ~244.2 g/mol · Solubility: Soluble in water. · Uses: Neuropsychiatric drug.
Uridine can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 95% |
|---|---|
| Buffer | None |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Pyrimidine Nucleoside |
| Analyzing Compounds | Uridine |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended