HPLC Method for Analysis of Tyramine on Primesep 100 Column on Cromite™
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Tyramine
Tyramine is an amine compound with the molecular formula C 8 H 11 NO . As a monoamine oxidase (MAO) substrate, it is broken down by the enzyme MAO in the body. It is typically found in fermented, aged, smoked, and dried foods.
Tyramine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Amine |
| Analyzing Compounds | Tyramine |
HPLC Column Used
Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended