HPLC Method for Analysis of Troger’s Base on Primesep 200 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Troger's base .
Troger’s base , also written as Tröger’s base , is a tetracyclic organic compound with the chemical formula C 17 H 18 N 2 . The correct structure for the compound was a mystery for 48 years after it’s initial synthesis by Julius Tröger. You can find detailed UV spectra of Troger's base and information about its various lambda maxima by visiting the following link.
Troger's base can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using LC MS.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | H2SO4– 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 |
| Limit Of Detection* | 80 ppb |
| Class of Compounds | Organic Acid |
| Analyzing Compounds | Troger's base |
HPLC Column Used
Primesep 200, 4.6 x 100 mm, 5 µm, 100 A, dual ended