HPLC Method for Analysis of trans-Zeatin on Primesep 100 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-trans-zeatinChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of trans-Zeatin
trans-Zeatin is a cytokinin derived from adenine with two forms, cis- and trans-isomer. It has the chemical formula C 10 H 13 N 5 O . It promotes callus initiation and fruit set, delays yellowing for vegetables, and causes auxiliary stems to grow. You can find detailed UV spectra of trans-Zeatin and information about its various lambda maxima by visiting the following link.
trans-Zeatin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with sulfuric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 40% |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Cytokinin |
| Analyzing Compounds | trans-Zeatin |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended