HPLC Method for Analysis of Thymol on Primesep 100 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-thymolChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol
Thymol ( 2-isopropyl-5-methylphenol ) is a natural monoterpenoid phenol derivative of p-Cymene. Thymol is the main monoterpene phenol occurring in essential oils isolated from plants such as the Thymus, Ocimum, Origanum, and other herbs with antimicrobial properties.
Thymol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 55% |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Monoterpenoid phenols |
| Analyzing Compounds | Thymol |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended