HPLC Method for Analysis of Thiamine Pyrophosphate and Pyridoxal Phosphate on Primesep SB Column

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Chromatogram

Method Parameters

Mobile PhaseMeCN
BufferAmFm pH 3.0
Flow Rate1.0 ml/min
DetectionUV, 290 nm
Class of CompoundsHydrophilic, Ionizable
Analyzing CompoundsThiamine Pyrophosphate, Pyridoxal Phosphate

HPLC Column Used

Primesep SB, 4.6×50 mm, 5 µm, 100A
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