HPLC Method for Analysis of Tetracycline on Primesep 100 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-tetracycline-2Chromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetracycline
Tetracycline is an antibiotic with the molecular formula C 22 H 24 N 2 O 8 . It is a bacteriostatic agent, therefore it prevents bacteria from growing and multiplying through inhibiting protein synthesis within bacteria. It is used to treat a variety of infections.
Tetracycline can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN – 20-80% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Medication |
| Analyzing Compounds | Tetracycline |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended