HPLC – MS Method for Analysis of Terbinafine on Primesep B Column
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HPLC - MS Method for Analysis of Terbinafine on Primesep B Column by SIELC Technologies
Description
Terbinafine is an antifungal medication commonly used to treat fungal infections, particularly those caused by dermatophytes. Here are some key points about terbinafine:
Mechanism of Action: Terbinafine is classified as an allylamine antifungal. It works by inhibiting the enzyme squalene epoxidase, which is important in the fungal ergosterol synthesis pathway. This disruption results in a deficiency of ergosterol in the fungal cell membrane and an accumulation of squalene, which is toxic to the fungus.
Terbinafine can be retained, and analyzed on a Primesep B mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected using UV at 283 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)
Method Parameters
| Mobile Phase | Gradient MeCN – 20-50%, 8 min |
|---|---|
| Buffer | Ammonium Formate pH 3.0-20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV, 282 nm, SIM 292+ |
| Class of Compounds | Allylamines |
| Analyzing Compounds | Terbinafine |