HPLC Method for Analysis of Tebuconazole on Newcrom B Column on Alltesta™
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Tebuconazole
Tebuconazole is a triazole fungicide with the chemical formula C 16 H 22 ClN 3 O . It is used against to plant pathogenic fungi. Despite the U.S. Food and Drug Administration considering this fungicide safe, it is considered a possible carcinogen with the rating of C. According to the World Health Organization, it has moderate toxicity and is slightly hazardous.
Tebuconazole can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Pesticide |
| Analyzing Compounds | Tebuconazole |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended