HPLC Method For Analysis Of Sulfamethoxazole and Trimethoprim on Primesep 100 Column

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Chromatogram

Description

· Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfamethoxazole and Trimethoprim

Method Parameters

Mobile PhaseMeCN/H2O – 40/60%
BufferH2SO4 – 0.4%
Flow Rate1.0 ml/min
DetectionUV, 200 nm,
Class of CompoundsDrug
Analyzing CompoundsSulfamethoxazole, Trimethoprim

HPLC Column Used

Primesep 100, Newcrom R1 4.6×150 mm, 5 µm, 100A