HPLC Method for Analysis of Sulfadiazine, Naphthylthiourea, Sulfamethazine, Dexamethasone, Cortisone 21-Acetate
https://sielc.com/hplc-method-for-analysis-of-sulfadiazine-naphthylthiourea-sulfamethazine-dexamethasone-cortisone-21-acetateChromatogram
Description
Sulfadiazine is an antibiotic used to prevent rheumatic fever, chancroid, chlamydia and infections by Haemophilus influenzae. There are side effects to the use of the drug which include, but not limited to: nausea, headache, rash, depression.
Method Parameters
| Mobile Phase | Gradient MeCN – 20-75%, 4 min |
|---|---|
| Buffer | Gradient H3PO4 – 0.05-0.2%, 4 min |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sulfadiazine, Naphthylthiourea, Sulfamethazine, Dexamethasone, Cortisone 21-Acetate |
HPLC Column Used
Primesep 100, 3.2×50 mm, 5 µm, 100A