ELSD HPLC Method for Analysis of Sucrose Stearate on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Sucrose Stearate .
Sucrose Stearate is a sugar ester with the chemical formula C 30 H 58 O 13 . It is used primarily as an emulsifier and moisturizer in lotions, creams, and cleansers.
Sucrose Stearate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of ethanol and acetonitrile (MeCN). Detection is performed using ELSD.
Method Parameters
| Mobile Phase | MeCN/EtOH |
|---|---|
| Buffer | None |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Class of Compounds | Sugar |
| Analyzing Compounds | Sucrose Stearate |
HPLC Column Used
Chromni, 3.2 x 100 mm, 3 µm, 100 A, dual ended